PU.1 / Spi1 Recombinant Rabbit Monoclonal Antibody [PD00-14]
cat.: HA721162
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PU.1 aa 1-100. |
| Positive control: | Daudi cell lysate, THP-1 cell lysate, human B-cell lymphoma tissue, Daudi. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:4,000 |
| Uniprot #: | SwissProt: P17947 Human |
| Alternative names: | transcription factor spi1 31 kDa Transforming Protein 31 kDa-transforming protein cb1086 Hematopoietic transcription factor PU.1 OF oncogene spi1 PU.1 SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of SFPI1 si:by184l24.2 SPI 1 SPI 1 proto oncogene SPI A Spi1 SPI1_HUMAN Spleen focus forming virus (SFFV) proviral integration oncogene spi1 Spleen focus forming virus proviral integration oncogene spi1 Transcription factor PU.1 |
Images
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Fig1:
Western blot analysis of PU.1 / Spi1 on different lysates with Rabbit anti-PU.1 / Spi1 antibody (HA721162) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721162) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-PU.1 / Spi1 antibody (HA721162) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721162) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.