TMEM16A Recombinant Rabbit Monoclonal Antibody [PO00-27]
cat.: HA721165
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PO00-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 114 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TMEM16A aa 400-500. |
| Positive control: | Human gastrointestinal stromal tumor tissue, human gallbladder tissue, human breast tissue. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
IHC-P |
1:1,000-1:1,500 |
| Uniprot #: | SwissProt: Q5XXA6 Human |
| Alternative names: | ANO 1 ANO1 ANO1_HUMAN Anoctamin 1 Anoctamin 1 calcium activated chloride channel Anoctamin-1 Anoctamin1 Ca2+ activated Cl- channel Calcium Activated Chloride Channel Discovered on gastrointestinal stromal tumors protein 1 DOG 1 DOG1 FLJ10261 Membrane protein Oral cancer overexpressed 2 Oral cancer overexpressed protein 2 ORAOV 2 ORAOV2 TAOS 2 TAOS2 TMEM 16A TMEM16A Transmembrane protein 16A (eight membrane spanning domains) Transmembrane protein 16A Tumor amplified and overexpressed sequence 2 Tumor-amplified and overexpressed sequence 2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.