KMT3B Recombinant Rabbit Monoclonal Antibody [1B1]
cat.: HA721166
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 1B1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 297 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KMT3B aa 1-200 / 2,696. |
| Positive control: | 293. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Cell |
1:100 |
| Uniprot #: | SwissProt: Q96L73 Human |
| Alternative names: | Androgen receptor coactivator 267 kDa protein Androgen receptor-associated protein of 267 kDa ARA267 H3 K36 HMTase H3-K36-HMTase H4 K20 HMTase H4-K20-HMTase Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific KMT3B Lysine N-methyltransferase 3B NR binding SET domain containing protein NR-binding SET domain-containing protein Nsd1 NSD1_HUMAN Nuclear receptor binding SET domain containing protein 1 Nuclear receptor binding SET domain protein 1 Nuclear receptor-binding SET domain-containing protein 1 |
Images
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Fig1:
Immunocytochemistry analysis of 293 cells labeling KMT3B with Rabbit anti-KMT3B antibody (HA721166) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KMT3B antibody (HA721166) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.