Human IL-6 Recombinant Rabbit Monoclonal Antibody [PS00-21]
cat.: HA721167
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PS00-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length of human IL6 protein. |
| Positive control: | Recombinant human IL6 protein. |
| Subcellular location: | Extracellular region or secreted. |
| Recommended Dilutions:
ELISA (Cap) |
Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P05231 Human Entrez Gene: 3569 Human Unigene: 654458 Human |
| Alternative names: | Interleukin BSF 2 B cell differentiation factor B cell stimulatory factor 2 B-cell stimulatory factor 2 BSF 2 BSF-2 BSF2 CDF CTL differentiation factor Cytotoxic T cell differentiation factor Hepatocyte stimulating factor Hepatocyte stimulatory factor HGF HSF Hybridoma growth factor Hybridoma growth factor Interferon beta-2 Hybridoma plasmacytoma growth factor IFN-beta-2 IFNB2 IL 6 IL-6 IL6 IL6_HUMAN Interferon beta 2 Interferon beta-2 Interleukin 6 Interleukin 6 (interferon beta 2) Interleukin BSF 2 Interleukin-6 |
Images
|
Fig1: Dilute the Capture Antibody (PS00-21) to the working concentration 2ug/ml in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at 4℃. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Add human IL6 protein with 1:3 serially diluted starting from a concentration of 1000pg/ml and incubate 45 minutes at room temperature. Add 100 μL of the Biotin-conjugated Detection Antibody (HA721168) at a dilution of 1:10,000 to each well. Cover with a new adhesive strip and incubate 45 minutes at room temperature. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 30 minutes at room temperature. Add 50 μL of Stop Solution to each well. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.