KDM3B Recombinant Rabbit Monoclonal Antibody [1A8]
cat.: HA721170
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 1A8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 191 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KDM3B aa 251-500. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, Hela, human testis tissue, human fallopian tube tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:100 1:400-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q7LBC6 Human | Q6ZPY7 Mouse |
| Alternative names: | 5qNCA C5orf7 JHDM2B JmjC domain containing histone demethylation protein 2B JmjC domain-containing histone demethylation protein 2B jmjd1b Jumonji domain containing 1B Jumonji domain containing protein 1B Jumonji domain-containing protein 1B KDM3B KDM3B_HUMAN KIAA1082 Lysine (K) specific demethylase 3B Lysine-specific demethylase 3B NET22 Nuclear protein 5qNCA |
Images
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Fig1:
Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA721170) at 1/1,000 dilution. Lane 1: Hela cell lysate (25 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Predicted band size: 191 kDa Observed band size: 191 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA721170) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA721170) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (HA721170) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721170) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-KDM3B antibody (HA721170) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721170) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of Hela cells labeling KDM3B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721170, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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