6X His-tag, AlpHcAbs Rabbit Alpaca Monoclonal Antibody
cat.: HA721172
| Product Type: | Alpaca monoclonal, primary antibodies |
|---|---|
| Applications: | WB, IP, ELISA |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS pH=7.5 |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Immunogen: | 6*His tag fusion protein. |
| Positive control: | C-terminal His-tag fusion protein lysate, N-terminal His-tag fusion protein lysate, Mid His-tag fusion protein lysate. |
| Recommended Dilutions:
WB WB WB IP ELISA |
1:10,000-1:50,000 (C-His) 1:500-1:10,000 (N-His) 1:10,000-1:50,000 (Mid-His) 1:100-1:500 1:10,000-1:50,000 |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Western blot analysis of 6X His-tag, AlpHcAbs Rabbit on different lysates with Rabbit anti-6X His-tag, AlpHcAbs Rabbit antibody (HA721172) at 1/50,000 dilution. Lane 1: C-terminal His-tag fusion protein lysate Lane 2: N-terminal His-tag fusion protein lysate Lane 3/4/5/6: Mid His-tag fusion protein lysate Lysates/proteins at 50 ng/Lane. Observed band size: 30/45/50 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721172) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA721172. Western blot was performed from the immunoprecipitate using HA721172 at 1/10,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal His-tag fusion protein lysate (input). Lane 2: HA721172 IP in C-terminal His-tag fusion protein lysate. Lane 3: Rabbit IgG instead of HA721172 IP in C-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST. |
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Fig3:
His-tag was immunoprecipitated in 2µg N-terminal His-tag fusion protein lysate with HA721172. Western blot was performed from the immunoprecipitate using HA721172 at 1/10,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 60 mins at room temperature. Lane 1: N-terminal His-tag fusion protein lysate (input). Lane 2: HA721172 IP in N-terminal His-tag fusion protein lysate. Lane 3: Rabbit IgG instead of HA721172 IP in N-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.