HER2 / ErbB2 Recombinant Rabbit Monoclonal Antibody [PD00-53]
cat.: HA721178
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, WB, IF-Cell, FC, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PD00-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 138 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ErbB2/ HER2 aa 500-650. |
| Positive control: | Human breast carcinoma tissue, SK-Br-3 cell lysates, MCF-7, SK-Br-3, human ovarian cancer tissue. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-P WB IF-Cell FC mIHC |
1:2,000 1:1,000 1:50 1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P04626 Human |
| Alternative names: | erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog C erb B2/neu protein CD340 CD340 antigen Cerb B2/neu protein CerbB2 Erb b2 receptor tyrosine kinase 2 ErbB-2 proto-oncogene ERBB2 ERBB2_HUMAN HER 2 HER 2/NEU HER2 Herstatin Human epidermal growth factor receptor 2 Metastatic lymph node gene 19 protein MLN 19 MLN19 NEU NEU proto oncogene Neuro/glioblastoma derived oncogene homolog Neuroblastoma/glioblastoma derived oncogene homolog NGL p185erbB2 Proto-oncogene c-ErbB-2 Proto-oncogene Neu Receptor tyrosine-protein kinase erbB-2 TKR1 Tyrosine kinase type cell surface receptor HER2 Tyrosine kinase-type cell surface receptor HER2 V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 V erb b2 avian erythroblastic leukemia viral oncoprotein 2 V erb b2 erythrobl...... |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721178) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721178) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of HER2 / ErbB2 on SK-Br-3 cell lysates with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of MCF-7 cells labeling HER2 / ErbB2 with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SK-Br-3 cells labeling HER2 / ErbB2 with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Flow cytometric analysis of SK-Br-3 cells labeling HER2 / ErbB2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721178, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7: mIHC analysis of human ovarian cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.