CSF-1-R Recombinant Rabbit Monoclonal Antibody [PD00-45]
cat.: HA721180
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P, FC
Clonality: Monoclonal
Clone number: PD00-45
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 108 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CSF1R aa 539-972 (Cytoplasmic)
Positive control: Human tonsil tissue, human colon carcinoma tissue.
Subcellular location: Cell membrane
Recommended Dilutions:
  IHC-P
  FC

1:2,000-1:10,000
1:1,000
Uniprot #: SwissProt: P07333 Human
Alternative names: C FMS CD 115 CD115 CD115 antigen CFMS Colony stimulating factor 1 receptor Colony stimulating factor I receptor CSF 1 R CSF 1R CSF-1 receptor CSF-1-R CSF1 R CSF1R CSF1R_HUMAN CSFR EC 2.7.10.1 FIM 2 FIM2 FMS FMS proto oncogene FMS protooncogene HDLS M-CSF Receptor M-CSF-R Macrophage colony stimulating factor 1 receptor Macrophage colony stimulating factor I receptor Macrophage colony-stimulating factor 1 receptor McDonough feline sarcoma viral (v fms) oncogene homolog MCSFR Oncogen FMS Proto-oncogene c-Fms V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
Images
HA721180_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721180_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721180_3.jpg Fig3: Flow cytometric analysis of SK-Br-3 cells labeling CSF-1-R.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721180, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.