Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | PD00-45 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 108 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human CSF1R aa 539-972 (Cytoplasmic) |
Positive control: | Human tonsil tissue, human colon carcinoma tissue. |
Subcellular location: | Cell membrane |
Recommended Dilutions:
IHC-P FC |
1:2,000-1:10,000 1:1,000 |
Uniprot #: | SwissProt: P07333 Human |
Alternative names: | C FMS CD 115 CD115 CD115 antigen CFMS Colony stimulating factor 1 receptor Colony stimulating factor I receptor CSF 1 R CSF 1R CSF-1 receptor CSF-1-R CSF1 R CSF1R CSF1R_HUMAN CSFR EC 2.7.10.1 FIM 2 FIM2 FMS FMS proto oncogene FMS protooncogene HDLS M-CSF Receptor M-CSF-R Macrophage colony stimulating factor 1 receptor Macrophage colony stimulating factor I receptor Macrophage colony-stimulating factor 1 receptor McDonough feline sarcoma viral (v fms) oncogene homolog MCSFR Oncogen FMS Proto-oncogene c-Fms V-FMS McDonough feline sarcoma viral oncogen homolog, formerly |
Fig1:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of SK-Br-3 cells labeling CSF-1-R. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721180, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |