HA721182

c-Myc Recombinant Rabbit Monoclonal Antibody [PS00-11]

cat.: HA721182

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IP, ChIP
Clonality:	Monoclonal
Clone number:	PS00-11

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 49 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide.
Positive control:	HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Jurkat cell lysate, Daudi cell lysate, K562 cell lysate, HL-60 cell lysate, human B lymphocytoma tissue, human colon tissue, human colon carcinoma tissue.
Subcellular location:	Nucleoplasm, nucleolus
Recommended Dilutions: WB IHC-P IP ChIP	1:1,000 1:200 1-2μg/sample Use 0.5~2 μg for 25 μg of chromatin.
Uniprot #:	SwissProt: P01106 Human \| P01108 Mouse \| P09416 Rat
Alternative names:	AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Cellular myelocytomatosis oncogene Class E basic helix-loop-helix protein 39 MGC105490 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 myca MYCC Myelocytomatosis oncogene a Myelocytomatosis oncogene Niard Nird oncogene c-Myc Oncogene Myc OTTHUMP00000158589 OTTHUMP00000227763 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) zc-myc

Images

Fig1: Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (HA721182) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 49 kDa
Observed band size: 55 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721182) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (HA721182) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: Daudi cell lysate (10 µg/Lane)
Lane 3: K562 cell lysate (10 µg/Lane)
Lane 4: HL-60 cell lysate (10 µg/Lane)

Predicted band size: 49 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes;
10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721182) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-c-Myc antibody (HA721182) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721182) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-c-Myc antibody (HA721182) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721182) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-c-Myc antibody (HA721182) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721182) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: c-Myc was immunoprecipitated from 0.2 mg HeLa cell lysate with HA721182 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721182 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA721182 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA721182 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 30 seconds; ECL: K1801

Fig7: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either c-Myc (HA721182) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.