Tbx3 Recombinant Rabbit Monoclonal Antibody [JE33-38]
cat.: HA721184
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tbx3 aa 694-743 / 743. |
| Positive control: | JAR cell lysates, HepG2. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: O15119 Human |
| Alternative names: | Bladder cancer related protein XHL T box 3 T-box protein 3 T-box transcription factor TBX3 Tbx3 TBX3 ISO TBX3_HUMAN UMS XHL |
Images
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Fig1:
Western blot analysis of Tbx3 on JAR cell lysates with Rabbit anti-Tbx3 antibody (HA721184) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721184) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HepG2 cells labeling Tbx3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721184, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.