EZH1 Recombinant Rabbit Monoclonal Antibody [PS01-09]
cat.: HA721187
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: PS01-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human EZH1 aa 471-510/747.
Positive control: HCT116 cell lysate, K562 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat large intestine tissue, human colon tissue, mouse large intestine tissue, HCT116.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1:2,000
1:200
Uniprot #: SwissProt: Q92800 Human | P70351 Mouse
Entrez Gene: 303547 Rat
Alternative names: Enhancer of zeste homolog 1 (Drosophila) Enhancer of zeste homolog 1 ENX-2 ENX2 Ezh1 EZH1_HUMAN Histone-lysine N-methyltransferase EZH1
Images
HA721187_1.jpg Fig1: Western blot analysis of EZH1 on different lysates with Rabbit anti-EZH1 antibody (HA721187) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate (20 µg/Lane)
Lane 2: K562 cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (17 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 85 kDa
Observed band size: 85/110 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721187) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721187_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-EZH1 antibody (HA721187) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721187) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721187_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EZH1 antibody (HA721187) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721187) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721187_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-EZH1 antibody (HA721187) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721187) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721187_5.jpg Fig5: Immunocytochemistry analysis of HCT116 cells labeling EZH1 with Rabbit anti-EZH1 antibody (HA721187) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EZH1 antibody (HA721187) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.