Phospho-eNOS (S1177) Recombinant Rabbit Monoclonal Antibody [PS01-10]
cat.: HA721188
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Dog, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PS01-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 133 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS. |
| Positive control: | HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate, human placenta tissue. |
| Subcellular location: | Cell membrane, Membrane, caveola, Cytoplasm, cytoskeleton, Golgi apparatus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P29474 Human |
| Alternative names: | cNOS Constitutive NOS EC NOS EC-NOS ecNOS Endothelial nitric oxidase synthase Endothelial nitric oxide synthase Endothelial nitric oxide synthase 3 Endothelial NOS eNOS Nitric oxide synthase 3 (endothelial cell) Nitric oxide synthase 3 Nitric oxide synthase 3 endothelial cell Nitric oxide synthase endothelial Nitric oxide synthase, endothelial NOS 3 NOS III NOS type III NOS3 NOS3_HUMAN NOSIII |
Images
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Fig1:
Western blot analysis of Phospho-eNOS (S1177) on different lysates with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/1,000 dilution. Lane 1: HAEC cell lysate Lane 2: HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 133 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721188) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721188) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.