Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18]
cat.: HA721190
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PS01-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 301 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr1989 of human ATR. |
| Positive control: | HeLa cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours. |
| Subcellular location: | Chromosome, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q13535 Human |
| Alternative names: | Ataxia telangiectasia and Rad3 related Ataxia telangiectasia and Rad3-related protein ATR ATR_HUMAN FCTCS FRAP Related Protein 1 FRAP-related protein 1 FRP1 MEC1 MEC1 mitosis entry checkpoint 1 homolog Protein kinase ATR RAC3 Rad3 related protein SCKL SCKL1 Serine/threonine protein kinase ATR Serine/threonine-protein kinase ATR |
Images
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Fig1:
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with Hydroxyurea cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721190) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells treated with or without 4mM Hydroxyurea for 20 hours labeling Phospho-ATR (T1989) with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/200 dilution. Image shown an increased nuclear staining upon Hydroxyurea treatment. Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS permeabilization for 5 minutes, and then blocked with 2% negative goat serum for 60 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/200 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.