NEDD4 Recombinant Rabbit Monoclonal Antibody [JE35-58]
cat.: HA721191
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment corresponding to Human NEDD4 aa 160-460. |
| Positive control: | U87MG cell lysate, A549 cell lysate, NIH/3T3 cell lysate, rat skin tissue lysate, human skin tissue lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P46934 Human | P46935 Mouse | Q62940 Rat |
| Alternative names: | Cell proliferation-inducing gene 53 protein E3 ubiquitin protein ligase Nedd4 E3 ubiquitin-protein ligase NEDD4 HECT-type E3 ubiquitin transferase NEDD4 KIAA0093 NEDD 4 NEDD-4 Nedd4 NEDD4_HUMAN Neural precursor cell expressed developmentally down regulated 4 Neural precursor cell expressed developmentally down-regulated protein 4 neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase PIG53 receptor-potentiating factor 1 RPF1 |
Images
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Fig1:
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/500 dilution. Lane 1: Rat skin tissue lysate Lane 2: Human skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.