delta 1 Catenin/CAS Recombinant Rabbit Monoclonal Antibody [PD00-43]
cat.: HA721193
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human delta 1 Catenin/CAS aa 850-950 (C terminal). |
| Positive control: | SK-Br-3 cell lysates, human breast cancer tissue, human liver cancer tissue, human prostate carcinoma tissue, human ovary carcinoma tissue, human esophagus tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:500-1:2,000 1:500 |
| Uniprot #: | SwissProt: O60716 Human |
| Alternative names: | Cadherin associated Src substrate Cadherin-associated Src substrate CAS Catenin (cadherin associated protein) delta 1 Catenin delta 1 Catenin delta Catenin delta-1 CTND1_HUMAN CTNND 1 CTNND CTNND1 delta 1 Catenin KIAA0384 p120 P120 CAS p120 catenin P120 CTN p120(cas) p120(ctn) P120CAS P120CTN |
Images
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Fig1:
Western blot analysis of delta 1 Catenin/CAS on SK-Br-3 cell lysates with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 108 kDa Observed band size: 95 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721193) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721193) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721193) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721193) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721193) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-delta 1 Catenin/CAS antibody (HA721193) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721193) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Application: IF-Tissue Species: Human Site: liver cancer Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.