ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]
cat.: HA721194
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 148 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ErbB3 aa 650-750 (internal sequence). |
| Positive control: | Human small intestine tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P21860 Human |
| Alternative names: | c erbB 3 c erbB3 Erb b2 receptor tyrosine kinase 3 ErbB 3 ERBB3 ERBB3 protein erbB3 S ERBB3_HUMAN Glial growth factor receptor HER 3 HER3 Human epidermal growth factor receptor 3 LCCS2 MDA BF 1 MGC88033 p180 ErbB3 p45 sErbB3 p85 sErbB3 proto-oncogene-like protein c ErbB 3 proto-oncogene-like protein c ErbB3 Proto-oncogene-like protein c-ErbB-3 Receptor tyrosine protein kinase erbB 3 Receptor tyrosine protein kinase erbB3 Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase type cell surface receptor HER3 Tyrosine kinase-type cell surface receptor HER3 v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) v erb b2 erythroblastic leukemia viral oncogene homolog 3 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling ErbB3/HER3 with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721194, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.