FOXP1 Recombinant Rabbit Monoclonal Antibody [PD00-47]
cat.: HA721195
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FOXP1 aa 350-450. |
| Positive control: | Human colon tissue, human lymph nodes tissue, human breast tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q9H334 Human |
| Alternative names: | 12CC4 FLJ23741 Fork head related protein like B Forkhead box P1 Forkhead box protein P1 FOX P1 FOXP 1 foxp1 FOXP1_HUMAN Glutamine rich factor 1 hFKH1B HSPC215 MGC12942 MGC88572 MGC99551 QRF 1 QRF1 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.