MAGEA1 Recombinant Rabbit Monoclonal Antibody [PD00-50]
cat.: HA721197
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C terminus Human MAGE-A1. |
| Positive control: | A431 cell lysates, human malignant melanoma tissue, human testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP IF-Tissue |
1:1,000 1:1,500 1-2μg/sample 1:200 |
| Uniprot #: | SwissProt: P43355 Human |
| Alternative names: | Antigen MZ2 E Antigen MZ2-E Cancer/testis antigen 1.1 cancer/testis antigen family 1, member 1 CT1.1 MAGA1_HUMAN MAGE 1 antigen MAGE-1 antigen MAGE1 MAGE1A MAGEA1 Melanoma antigen 1 Melanoma antigen family A 1 (directs expression of antigen MZ2 E) Melanoma antigen family A 1 Melanoma antigen MAGE 1 Melanoma associated antigen 1 Melanoma associated antigen MZ2 E Melanoma-associated antigen 1 MGC9326 |
Images
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Fig1:
Western blot analysis of MAGEA1 on A431 cell lysates with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
MAGEA1 was immunoprecipitated from 0.2 mg A431 cell lysate with HA721197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721197 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA721197 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA721197 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801 |
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Fig5:
Application: IF-Tissue Species: Human Site: testis Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.