Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | PD00-51 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: |
Isotype: | IgG |
Immunogen: | Synthetic peptide. |
Positive control: | Human bladder carcinoma tissue, human colon carcinoma tissue, human stomach carcinoma tissue. |
Subcellular location: | Chromosome, Cytoplasm, Nucleus. |
Recommended Dilutions:
IHC-P |
1:500 |
Uniprot #: | SwissProt: Q969H0 Human |
Alternative names: | AGO Archipelago Archipelago homolog Archipelago, Drosophila, homolog of CDC4 CDC4, S.cerevisiae, homolog of DKFZp686F23254 F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) F box and WD 40 domain protein 7 F box and WD repeat domain containing 7 F box protein FBW7 F box protein FBX30 F box protein SEL 10 F box protein SEL10 F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase F-box and WD-40 domain-containing protein 7 F-box protein FBX30 F-box/WD repeat-containing protein 7 FBW6 FBW7 FBX30 FBXO30 FBXW6 FBXW7 FBXW7_HUMAN FLJ16457 hAgo hCdc4 Homolog of C elegans sel 10 Homolog of C.elegans sel10 SEL 10 SEL-10 SEL10 |
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Fig1:
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |