ALDH3A1 Recombinant Rabbit Monoclonal Antibody [PD00-73]
cat.: HA721201
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide near the C-terminus of human ALDH3A1 protein. |
| Positive control: | A549 cell lysate, human skin tissue lysate, human lung tissue, human esophagus tissue, human stomach tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P30838 Human |
| Alternative names: | Acetaldehyde dehydrogenase 3 AHD 4 AHD C AHD4 AHDC AL3A1_HUMAN Aldehyde dehydrogenase 3 Aldehyde dehydrogenase 3 family member A1 Aldehyde dehydrogenase 3A1 Aldehyde dehydrogenase Aldehyde dehydrogenase class 3 Aldehyde dehydrogenase dimeric NADP preferring Aldehyde dehydrogenase family 3 member A1 Aldehyde dehydrogenase family 3 subfamily A1 Aldehyde dehydrogenase family 3, subfamily A, member 1 Aldehyde dehydrogenase isozyme 3 Aldehyde dehydrogenase type III ALDH 3 ALDH 3A1 ALDH III ALDH, stomach type ALDH3 A1 ALDH3 ALDH3A 1 Aldh3a1 ALDHIII dimeric NADP-preferring MGC10406 Stomach aldehyde dehydrogenase Tumor associated aldehyde dehydrogenase tumor ALDH |
Images
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Fig1:
Western blot analysis of ALDH3A1 on different lysates with Rabbit anti-ALDH3A1 antibody (HA721201) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: Human skin tissue lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ALDH3A1 antibody (HA721201) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721201) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-ALDH3A1 antibody (HA721201) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721201) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ALDH3A1 antibody (HA721201) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721201) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.