ENT1 Recombinant Rabbit Monoclonal Antibody [PD00-77]
cat.: HA721203
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: PD00-77
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminus of human SLC29A1.
Positive control: Human pancreas tissue, human colon carcinoma tissue, human placenta tissue.
Subcellular location: Basolateral cell membrane, Apical cell membrane, Cell membrane.
Recommended Dilutions:
  IHC-P

1:200-1:1,000
Uniprot #: SwissProt: Q99808 Human
Alternative names: Equilibrative NBMPR-sensitive nucleoside transporter equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter Equilibrative nucleoside transporter 1 es-type MGC1465 MGC3778 Nucleoside transporter Nucleoside transporter, es-type OTTHUMP00000016506 OTTHUMP00000016507 OTTHUMP00000016508 OTTHUMP00000016509 OTTHUMP00000016510 OTTHUMP00000016511 OTTHUMP00000016512 S29A1_HUMAN Slc29a1 solute carrier family 29 (equilibrative nucleoside transporter), member 1 solute carrier family 29 (nucleoside transporters), member 1 Solute carrier family 29 member 1
Images
HA721203_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721203_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721203_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.