Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | PD00-77 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 50 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C-terminus of human SLC29A1. |
Positive control: | Human pancreas tissue, human colon carcinoma tissue, human placenta tissue. |
Subcellular location: | Basolateral cell membrane, Apical cell membrane, Cell membrane. |
Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
Uniprot #: | SwissProt: Q99808 Human |
Alternative names: | Equilibrative NBMPR-sensitive nucleoside transporter equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter Equilibrative nucleoside transporter 1 es-type MGC1465 MGC3778 Nucleoside transporter Nucleoside transporter, es-type OTTHUMP00000016506 OTTHUMP00000016507 OTTHUMP00000016508 OTTHUMP00000016509 OTTHUMP00000016510 OTTHUMP00000016511 OTTHUMP00000016512 S29A1_HUMAN Slc29a1 solute carrier family 29 (equilibrative nucleoside transporter), member 1 solute carrier family 29 (nucleoside transporters), member 1 Solute carrier family 29 member 1 |
Fig1:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ENT1 antibody (HA721203) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721203) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |