| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-87 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein corresponding to Human CTAG1B. |
| Positive control: | Human testis tissue, human stomach carcinoma tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IHC-P |
1:2,000 |
| Uniprot #: | SwissProt: P78358 Human |
| Alternative names: | CTAG1A CTAG1B Autoimmunogenic cancer/testis antigen NY ESO 1 Autoimmunogenic cancer/testis antigen NY-ESO-1 Cancer antigen 3 Cancer/testis antigen 1 Cancer/testis antigen 1B Cancer/testis antigen 6.1 CT6.1 CTAG 1 CTAG 1B CTAG CTAG1 CTAG1B CTG1B_HUMAN ESO 1 ESO1 L antigen family member 2 LAGE 2 LAGE 2 protein LAGE 2B LAGE-2 LAGE2 LAGE2 protein LAGE2A LAGE2B New York esophageal squamous cell carcinoma 1 NY ESO 1 NYESO 1 NYESO1 |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CTAG1B antibody (HA721204) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721204) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-CTAG1B antibody (HA721204) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721204) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |