Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | PD00-87 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 18 kDa |
Isotype: | IgG |
Immunogen: | Recombinant full length protein corresponding to Human CTAG1B. |
Positive control: | Human testis tissue, human stomach carcinoma tissue. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
IHC-P |
1:2,000 |
Uniprot #: | SwissProt: P78358 Human |
Alternative names: | CTAG1A CTAG1B Autoimmunogenic cancer/testis antigen NY ESO 1 Autoimmunogenic cancer/testis antigen NY-ESO-1 Cancer antigen 3 Cancer/testis antigen 1 Cancer/testis antigen 1B Cancer/testis antigen 6.1 CT6.1 CTAG 1 CTAG 1B CTAG CTAG1 CTAG1B CTG1B_HUMAN ESO 1 ESO1 L antigen family member 2 LAGE 2 LAGE 2 protein LAGE 2B LAGE-2 LAGE2 LAGE2 protein LAGE2A LAGE2B New York esophageal squamous cell carcinoma 1 NY ESO 1 NYESO 1 NYESO1 |
Fig1:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CTAG1B antibody (HA721204) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721204) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-CTAG1B antibody (HA721204) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721204) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |