IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]
cat.: HA721205
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P, FC
Clonality: Monoclonal
Clone number: PD00-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human IGJ aa 50-150 (internal sequence).
Positive control: Human tonsil tissue, Daudi.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P
  FC

1:2,000
1:500-1:1,000
Uniprot #: SwissProt: P01591 Human
Alternative names: IGCJ IGJ_HUMAN Immunoglobulin J chain Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides immunoglobulin joining chain JCH JCHAIN Joining chain of multimeric IgA and IgM
Images
HA721205_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGJ antibody (HA721205) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721205) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721205_2.jpg Fig2: Flow cytometric analysis of Daudi cells labeling IGJ.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721205, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.