Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | PD00-91 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 33 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Nucleophosmin aa 1-100 (N terminal). |
Positive control: | Hela cell lysate, HepG2 cell lysate, A431 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, rat kidney tissue, mouse small intestine tissue, LOVO, Daudi, HL-60, human colon carcinoma tissue, human skin tissue. |
Subcellular location: | Nucleus, nucleolus, nucleoplasm, cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50 1:200-1:500 1:500-1:1,000 |
Uniprot #: | SwissProt: P06748 Human | Q61937 Mouse | P13084 Rat |
Alternative names: | B23 MGC104254 NO38 NPM NPM_HUMAN NPM1 Nucleolar phosphoprotein B23 Nucleolar protein NO38 Nucleophosmin (nucleolar phosphoprotein B23 numatrin) Nucleophosmin nucleophosmin nucleoplasmin family member 1 Nucleophosmin/nucleoplasmin family member 1 Numatrin OTTHUMP00000161024 OTTHUMP00000161025 OTTHUMP00000223397 OTTHUMP00000223398 |
Fig1:
Western blot analysis of Nucleophosmin on different lysates with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: C2C12 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721206) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of LOVO cells labeling Nucleophosmin with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721206) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721206) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721206) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig6:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Nucleophosmin antibody (HA721206) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721206) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of Daudi cells labeling Nucleophosmin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721206, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig8:
Flow cytometric analysis of HL-60 cells labeling Nucleophosmin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721206, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |