Tcl1 Recombinant Rabbit Monoclonal Antibody [PD00-94]
cat.: HA721207
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-94 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Tcl1 aa 1-114. |
| Positive control: | Daudi cell lysate, Ramos cell lysate, human tonsil tissue, human appendix tissue, PC-3, Daudi. |
| Subcellular location: | Cytoplasm, Nucleus, Microsome, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC IF-Tissue |
1:1,000 1:10,000-1:30,000 1:50 1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P56279 Human |
| Alternative names: | Anti TCL1A Lymphoma/leukemia, T-cell Oncogene TCL-1 Oncogene TCL1 P14 TCL1 P14 TCL1 protein Protein p14 TCL1 T cell leukemia 1 T cell lymphoma 1 T cell lymphoma 1A T-cell leukemia/lymphoma 1A T-cell leukemia/lymphoma protein 1A TCL 1 protein TCL1 TCL1 oncogene TCL1 PEN Tcl1a TCL1A TCL1A_HUMAN |
Images
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Fig1:
Western blot analysis of Tcl1 on different lysates with Rabbit anti-Tcl1 antibody (HA721207) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Ramos cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA721207, 1/30,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Immunocytochemistry analysis of PC-3 cells labeling Tcl1 with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of Daudi cells labeling Tcl1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Application: IF-Tissue Species: Human Site: appendix Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.