Tcl1 Recombinant Rabbit Monoclonal Antibody [PD00-94]
cat.: HA721207
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: PD00-94
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 13 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Tcl1 aa 1-114.
Positive control: Daudi cell lysate, Raji cell lysate, human tonsil tissue, human appendix tissue, PC-3, Daudi.
Subcellular location: Cytoplasm, Nucleus, Microsome, Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:1,000
1:2,000
1:50
1:500-1:1,000
Uniprot #: SwissProt: P56279 Human
Alternative names: Anti TCL1A Lymphoma/leukemia, T-cell Oncogene TCL-1 Oncogene TCL1 P14 TCL1 P14 TCL1 protein Protein p14 TCL1 T cell leukemia 1 T cell lymphoma 1 T cell lymphoma 1A T-cell leukemia/lymphoma 1A T-cell leukemia/lymphoma protein 1A TCL 1 protein TCL1 TCL1 oncogene TCL1 PEN Tcl1a TCL1A TCL1A_HUMAN
Images
HA721207_1.jpg Fig1: Western blot analysis of Tcl1 on different lysates with Rabbit anti-Tcl1 antibody (HA721207) at 1/1,000 dilution.

Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 13 kDa
Observed band size: 13 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721207_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Tcl1 antibody (HA721207) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721207) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721207_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Tcl1 antibody (HA721207) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721207) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721207_4.jpg Fig4: Immunocytochemistry analysis of PC-3 cells labeling Tcl1 with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721207_5.jpg Fig5: Flow cytometric analysis of Daudi cells labeling Tcl1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.