HA721209

VPAC1 Recombinant Rabbit Monoclonal Antibody [PD00-96]

cat.: HA721209

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey
Applications:	IHC-P, WB
Clonality:	Monoclonal
Clone number:	PD00-96

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 52 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human VIPR1 aa 400-457.
Positive control:	HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, HT-29 cell lysate, Caco-2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, PC-12 cell lysate, LNCaP cell lysate, human tonsil tissue, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, C6 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, mouse small intestine tissue lysate, mouse colon tissue lysate, rat lung tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, rat spleen tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: IHC-P WB	1:500-1:4,000 1:1,000
Uniprot #:	SwissProt: P32241 Human \| P97751 Mouse \| P30083 Rat
Alternative names:	FLJ41949 HVR 1 HVR1 PACAP R 2 PACAP type II receptor PACAP-R-2 PACAP-R2 Pituitary adenylate cyclase activating polypeptide receptor type II Pituitary adenylate cyclase activating polypeptide type II receptor Pituitary adenylate cyclase-activating polypeptide type II receptor RCD1 RDC 1 RDC1 V1RG VAPC 1 VAPC1 Vasoactive intestinal peptide receptor 1 Vasoactive intestinal polypeptide receptor 1 Vasoactive intestinal polypeptide receptor 1 precursor VIP and PACAP receptor 1 VIP R 1 VIP receptor type I VIP-R-1 VIPR 1 VIPR VIPR1 VIPR1_HUMAN VIRG VPAC 1 VPAC1 VPCAP1R

Images

Fig1: Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Caco-2 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: LNCaP cell lysate (20 µg/Lane)

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721209) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig3: Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: C2C12 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: L6 cell lysate (20 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Mouse small intestine tissue lysate (40 µg/Lane)
Lane 9: Mouse colon tissue lysate (40 µg/Lane)
Lane 10: Rat lung tissue lysate (40 µg/Lane)
Lane 11: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 1 minute 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721209) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.