HER2 / ErbB2 Recombinant Rabbit Monoclonal Antibody [PD00-99]
cat.: HA721210
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 138 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within Human ErbB2/ HER2 aa 500-650. |
| Positive control: | SK-Br-3 cell lysates, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:4,000 1:200 |
| Uniprot #: | SwissProt: P04626 Human |
| Alternative names: | erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog C erb B2/neu protein CD340 CD340 antigen Cerb B2/neu protein CerbB2 Erb b2 receptor tyrosine kinase 2 ErbB-2 proto-oncogene ERBB2 ERBB2_HUMAN HER 2 HER 2/NEU HER2 Herstatin Human epidermal growth factor receptor 2 Metastatic lymph node gene 19 protein MLN 19 MLN19 NEU NEU proto oncogene Neuro/glioblastoma derived oncogene homolog Neuroblastoma/glioblastoma derived oncogene homolog NGL p185erbB2 Proto-oncogene c-ErbB-2 Proto-oncogene Neu Receptor tyrosine-protein kinase erbB-2 TKR1 Tyrosine kinase type cell surface receptor HER2 Tyrosine kinase-type cell surface receptor HER2 V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 V erb b2 avian erythroblastic leukemia viral oncoprotein 2 V erb b2 erythrobl...... |
Images
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Fig1:
Western blot analysis of HER2 / ErbB2 on SK-Br-3 cell lysates with Rabbit anti-HER2 / ErbB2 antibody (HA721210) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721210) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HER2 / ErbB2 antibody (HA721210) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721210) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling HER2 / ErbB2 (HA721210, green) and Histone H3 (HA601335, red). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies HER2 / ErbB2 (HA721210, green) at 1/200 dilution and Histone H3 (HA601335, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Rabbit IgG (HA1121) and iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.