LMO2 Recombinant Rabbit Monoclonal Antibody [PD00-25]
cat.: HA721212
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein full length human LMO2. |
| Positive control: | K562 cell lysates, mouse brain tissue, human tonsil tissue, human lymph nodes tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000-1:2000 |
| Uniprot #: | SwissProt: P25791 Human | P25801 Mouse |
| Alternative names: | Cysteine rich protein TTG 2 Cysteine rich protein TTG2 Cysteine-rich protein TTG-2 LIM domain only 2 (rhombotin like 1) LIM domain only 2 LIM domain only protein 2 LMO 2 LMO-2 lmo2 RBTN 2 RBTN L1 RBTN2 RBTN2_HUMAN RBTNL 1 RBTNL1 RHOM 2 RHOM2 Rhombotin 2 Rhombotin like 1 Rhombotin-2 Rhombotin2 T cell translocation gene 2 T cell translocation protein 2 T-cell translocation protein 2 TTG 2 TTG2 |
Images
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Fig1:
Western blot analysis of LMO2 on K562 cell lysates with Rabbit anti-LMO2 antibody (HA721212) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721212) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.