CA125/MUC16 Recombinant Rabbit Monoclonal Antibody [PD01-13]
cat.: HA721217
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, WB, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD01-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 1,519 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | Human ovarian serous carcinoma tissue, human endometrium tissue, human ovary cancer tissue, NIH:OVCAR-3 cell lysate, HeLa cell lysate. |
| Subcellular location: | Cell membrane, secreted, extracellular space. |
| Recommended Dilutions:
IHC-P WB IF-Tissue |
1:1,000-1:2,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q8WXI7 Human |
| Alternative names: | CA 125 CA-125 CA125 CA125 ovarian cancer antigen Cancer antigen 125 FLJ14303 MUC 16 MUC-16 MUC16 MUC16_HUMAN Mucin 16 Mucin 16 cell surface associated Mucin-16 Mucin16 Ovarian cancer related tumor marker CA125 Ovarian cancer-related tumor marker CA125 Ovarian carcinoma antigen CA125 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunocytochemistry analysis of SKOV-3 cells labeling CA125/MUC16 with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of CA125/MUC16 on different lysates with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/1,000 dilution. Lane 1: NIH:OVCAR-3 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 1519 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721217) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig7:
Application: IF-Tissue Species: Human Site: ovarian serous carcinoma Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.