NAPSIN A Recombinant Rabbit Monoclonal Antibody [PD01-30]
cat.: HA721218
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: PD01-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Recombinant fragment within Human NAPSIN A aa 1-150.
Positive control: Human lung carcinoma tissue, human kidney tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P

1:1,000
Uniprot #: SwissProt: O96009 Human
Alternative names: Asp 4 ASP4 Aspartyl protease 4 KAP Kdap Kidney derived aspartic protease like protein NAP1 NAPA Napsa NAPSA_HUMAN Napsin 1 napsin A aspartic peptidase Napsin A precursor Napsin-1 Napsin-A Pronapsin A SNAPA TA01/TA02
Images
HA721218_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-NAPSIN A antibody (HA721218) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721218) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721218_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NAPSIN A antibody (HA721218) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721218) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721218_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-NAPSIN A antibody (HA721218) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721218) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.