| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 9 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein corresponding to Human SPINK1/P12 aa 1 to the C-terminus. |
| Positive control: | Human pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:2,000 |
| Uniprot #: | SwissProt: P00995 Human |
| Alternative names: | ISK1_HUMAN Pancreatic secretory trypsin inhibitor Pancreatic serine protease inhibitor Kazal PCTT PSTI Serine protease inhibitor Kazal type 1 Serine protease inhibitor Kazal-type 1 Spink 3 SPINK1 Spink3 TaTI TCP Tumor associated trypsin inhibitor Tumor-associated trypsin inhibitor |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SPINK1 antibody (HA721221) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721221) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |