hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01]
cat.: HA721224
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PH00-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 186 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C terminal human SET1A. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, human lung carcinoma tissue, human testis tissue, mouse hippocampus tissue. |
| Subcellular location: | Chromosome, Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:400 |
| Uniprot #: | SwissProt: O15047 Human | E9PYH6 Mouse | A0A8I6AS32 Rat |
| Alternative names: | Histone-lysine N-methyltransferase SETD1A hSET1A KMT2F Lysine N methyltransferase 2F Lysine N-methyltransferase 2F SET domain containing 1A SET domain containing protein 1A SET domain-containing protein 1A SET1 Set1 Ash2 histone methyltransferase complex subunit SET1 Set1/Ash2 histone methyltransferase complex subunit SET1 SET1A SET1A_HUMAN SETD1A |
Images
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Fig1:
Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 186 kDa Observed band size: 300 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721224) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.