DPTOR Recombinant Rabbit Monoclonal Antibody [PH00-03]
cat.: HA721225
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PH00-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DPTOR aa 1-300. |
| Positive control: | MCF-7 cell lysate, Daudi cell lysate, PC-3M cell lysate, N2A cell lysate, L6 cell lysate, mouse brain tissue lysate, human small intestine tissue. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q8TB45 Human | Q570Y9 Mouse |
| Alternative names: | DEP domain containing 6 DEP domain-containing mTOR-interacting protein DEP domain-containing protein 6 DEP.6 DEPDC6 DEPTOR DPTOR_HUMAN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DPTOR antibody (HA721225) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721225) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of DPTOR on different lysates with Rabbit anti-DPTOR antibody (HA721225) at 1/1,000 dilution. Lane 1: Daudi (Human liver cancer cell) cell lysate Lane 2: NIH/3T3 (Human Burkitt's lymphoma cells) cell lysate Lane 3: L6 (Rat skeletal muscle myoblasts) cell lysate Lane 4: mouse heart tissue lysate Lysates/proteins at cell:20,ug/lane,tissue:40,ug/lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721225, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46.3 kDa Observed band size: 46 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.