DPTOR Recombinant Rabbit Monoclonal Antibody [PH00-03]
cat.: HA721225
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PH00-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DPTOR aa 1-300. |
| Positive control: | MCF-7 cell lysate, Daudi cell lysate, PC-3M cell lysate, N2A cell lysate, L6 cell lysate, mouse brain tissue lysate, human small intestine tissue. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q8TB45 Human | Q570Y9 Mouse |
| Alternative names: | DEP domain containing 6 DEP domain-containing mTOR-interacting protein DEP domain-containing protein 6 DEP.6 DEPDC6 DEPTOR DPTOR_HUMAN |
Images
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Fig1:
Western blot analysis of DPTOR on different lysates with Rabbit anti-DPTOR antibody (HA721225) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (20 µg/Lane) Lane 2: Daudi cell lysate (10 µg/Lane) Lane 3: PC-3M cell lysate (17 µg/Lane) Lane 4: N2A cell lysate (15 µg/Lane) Lane 5: L6 cell lysate (13 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 10 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721225) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DPTOR antibody (HA721225) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721225) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.