hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-02]
cat.: HA721226
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: PH00-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 186 kDa
Isotype: IgG
Immunogen: Recombinant protein within C terminal human SET1A.
Positive control: 293T cell lysates, 293T, HepG2.
Subcellular location: Chromosome, Nucleus
Recommended Dilutions:
  WB
  FC

1:1,000
1:500-1:1,000
Uniprot #: SwissProt: O15047 Human | E9PYH6 Mouse | A0A8I6AS32 Rat
Alternative names: Histone-lysine N-methyltransferase SETD1A hSET1A KMT2F Lysine N methyltransferase 2F Lysine N-methyltransferase 2F SET domain containing 1A SET domain containing protein 1A SET domain-containing protein 1A SET1 Set1 Ash2 histone methyltransferase complex subunit SET1 Set1/Ash2 histone methyltransferase complex subunit SET1 SET1A SET1A_HUMAN SETD1A
Images
HA721226_1.jpg Fig1: Western blot analysis of hSET1/SET1 on 293T cell lysates with Rabbit anti-hSET1/SET1 antibody (HA721226) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 186 kDa
Observed band size: 300 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721226) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721226_2.jpg Fig2: Flow cytometric analysis of 293T cells labeling hSET1/SET1.

Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721226_3.jpg Fig3: Flow cytometric analysis of HepG2 cells labeling hSET1/SET1.

Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.