Kir2.1 Recombinant Rabbit Monoclonal Antibody [JE54-56]
cat.: HA721227
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Kir2.1 aa 91-140 / 427. |
| Positive control: | HEK-293 cell lysate, A549 cell lysate,Neuro-2a cell lysate,PC-12 cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P63252 Human | P35561 Mouse | Q64273 Rat |
| Alternative names: | Cardiac inward rectifier potassium channel HHBIRK 1 HHBIRK1 HHIRK 1 HHIRK1 HIRK 1 hIRK1 Inward rectifier K Inward rectifier K(+) channel Kir2.1 Inward rectifier potassium channel 2 inwardly rectifying subfamily J member 2 IRK 1 IRK-1 IRK1 IRK2_HUMAN KCNJ2 KIR2.1 LQT 7 LQT7 Potassium channel Potassium channel inwardly rectifying subfamily J member 2 Potassium inwardly rectifying channel J2 Potassium inwardly rectifying channel subfamily J member 2 SQT 3 SQT3 |
Images
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Fig1:
Western blot analysis of Kir2.1 on different lysates with Rabbit anti-Kir2.1 antibody (HA721227) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721227, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.