Kir2.1 Recombinant Rabbit Monoclonal Antibody [JE54-56]
cat.: HA721227
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE54-56
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Kir2.1 aa 91-140 / 427.
Positive control: 293 cell lysates.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P63252 Human | P35561 Mouse | Q64273 Rat
Alternative names: Cardiac inward rectifier potassium channel HHBIRK 1 HHBIRK1 HHIRK 1 HHIRK1 HIRK 1 hIRK1 Inward rectifier K Inward rectifier K(+) channel Kir2.1 Inward rectifier potassium channel 2 inwardly rectifying subfamily J member 2 IRK 1 IRK-1 IRK1 IRK2_HUMAN KCNJ2 KIR2.1 LQT 7 LQT7 Potassium channel Potassium channel inwardly rectifying subfamily J member 2 Potassium inwardly rectifying channel J2 Potassium inwardly rectifying channel subfamily J member 2 SQT 3 SQT3
Images
HA721227_1.jpg Fig1: Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (HA721227) at 1/1,000 dilution.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 30s;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721227) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.