| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Kir2.1 aa 91-140 / 427. |
| Positive control: | 293 cell lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P63252 Human | P35561 Mouse | Q64273 Rat |
| Alternative names: | Cardiac inward rectifier potassium channel HHBIRK 1 HHBIRK1 HHIRK 1 HHIRK1 HIRK 1 hIRK1 Inward rectifier K Inward rectifier K(+) channel Kir2.1 Inward rectifier potassium channel 2 inwardly rectifying subfamily J member 2 IRK 1 IRK-1 IRK1 IRK2_HUMAN KCNJ2 KIR2.1 LQT 7 LQT7 Potassium channel Potassium channel inwardly rectifying subfamily J member 2 Potassium inwardly rectifying channel J2 Potassium inwardly rectifying channel subfamily J member 2 SQT 3 SQT3 |
|
Fig1:
Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (HA721227) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721227) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |