Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE50-55 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 112 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Ube1L aa 1-100 / 1,012. |
Positive control: | THP-1 cell lysates, rat kidney tissue, human testis tissue, mouse cerebellum tissue. |
Subcellular location: | Cytoplasm, cytosol, nucleoplasm, nucleus |
Recommended Dilutions:
WB IHC-P |
1:500 1:1,000 |
Uniprot #: | SwissProt: P41226 Human Entrez Gene: 74153 Mouse | 301000 Rat |
Alternative names: | D8 MGC12713 UBA1, ubiquitin activating enzyme E1 homolog B UBA1B UBA7 UBA7, ubiquitin activating enzyme E1 UBA7_HUMAN Ube 1L UBE 2 UBE1L UBE2 Ubiquitin activating enzyme 2 Ubiquitin activating enzyme E1 homolog Ubiquitin Activating Enzyme E1 Like Ubiquitin activating enzyme E1 related protein Ubiquitin-activating enzyme 7 Ubiquitin-activating enzyme E1 homolog ubiquitin-activating enzyme-2 ubiquitin-like modifier activating enzyme 7 Ubiquitin-like modifier-activating enzyme 7 |
Fig1:
Western blot analysis of Ube1L on THP-1 cell lysates with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 112 kDa Observed band size: 112 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721228) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |