Ube1L Recombinant Rabbit Monoclonal Antibody [JE50-55]
cat.: HA721228
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE50-55
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 112 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Ube1L aa 1-100 / 1,012.
Positive control: THP-1 cell lysates, rat kidney tissue, human testis tissue, mouse cerebellum tissue.
Subcellular location: Cytoplasm, cytosol, nucleoplasm, nucleus
Recommended Dilutions:
  WB
  IHC-P

1:500
1:1,000
Uniprot #: SwissProt: P41226 Human
Entrez Gene: 74153 Mouse | 301000 Rat
Alternative names: D8 MGC12713 UBA1, ubiquitin activating enzyme E1 homolog B UBA1B UBA7 UBA7, ubiquitin activating enzyme E1 UBA7_HUMAN Ube 1L UBE 2 UBE1L UBE2 Ubiquitin activating enzyme 2 Ubiquitin activating enzyme E1 homolog Ubiquitin Activating Enzyme E1 Like Ubiquitin activating enzyme E1 related protein Ubiquitin-activating enzyme 7 Ubiquitin-activating enzyme E1 homolog ubiquitin-activating enzyme-2 ubiquitin-like modifier activating enzyme 7 Ubiquitin-like modifier-activating enzyme 7
Images
HA721228_1.jpg Fig1: Western blot analysis of Ube1L on THP-1 cell lysates with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 112 kDa
Observed band size: 112 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721228) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721228_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721228_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721228_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Ube1L antibody (HA721228) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.