Human IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-20]
cat.: HA721232
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PS01-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein. |
| Positive control: | Recombinant IL-17A protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q16552 Human |
| Alternative names: | CTLA 8 CTLA-8 CTLA8 cytotoxic T lymphocyte associated antigen 8 Cytotoxic T lymphocyte associated protein 8 Cytotoxic T lymphocyte associated serine esterase 8 Cytotoxic T-lymphocyte-associated antigen 8 IL 17 IL 17A IL-17 IL-17A IL17 IL17_HUMAN Il17a Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) interleukin 17A Interleukin-17A interleukin17 Interleukin17A OTTHUMP00000016597 OTTMUSP00000046003 |
Images
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Fig1:
Sandwich ELISA analysis of IL-17A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721232 [PS01-20] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-17A protein (HA210731) starting from 2,000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721233 [PS01-24] (Biotin, 1/2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-17A protein, Streptavidin-HRP are diluted in 1%BSA/PBST. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.