Smad4 Recombinant Rabbit Monoclonal Antibody [PD01-37]
cat.: HA721234
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PD01-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Smad4 aa 500 to the C-terminus (C terminal). |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, PANC-1, human lung carcinoma tissue, human testis tissue, human stomach carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:50 |
| Uniprot #: | SwissProt: Q13485 Human | P97471 Mouse | O70437 Rat |
| Alternative names: | (Small) Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma 4 Deleted in Pancreatic Carcinoma Deleted in pancreatic carcinoma locus 4 Deletion target in pancreatic carcinoma 4 DPC 4 DPC4 hSMAD4 JIP MAD homolog 4 MAD mothers against decapentaplegic Drosophila homolog 4 MAD mothers against decapentaplegic homolog 4 MADH 4 MADH4 Med Medea Mothers against decapentaplegic homolog 4 Mothers against decapentaplegic, Drosophila, homolog of, 4 Mothers against DPP homolog 4 MYHRS OTTHUMP00000163548 SMA- and MAD-related protein 4 SMAD 4 SMAD family member 4 SMAD mothers against DPP homolog 4 SMAD4 SMAD4_HUMAN |
Images
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Fig1:
Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721234) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PANC-1 cells labeling Smad4 with Rabbit anti-Smad4 antibody (HA721234) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Smad4 antibody (HA721234) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.