AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
cat.: HA721238
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein corresponding to Human alpha 1 Fetoprotein. |
| Positive control: | Human liver carcinoma tissue, human placenta tissue. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: P02771 Human |
| Alternative names: | Afp AFPD Alpha fetoglobulin Alpha fetoprotein Alpha fetoprotein precursor Alpha-1-fetoprotein Alpha-fetoglobulin Alpha-fetoprotein alpha-fetoprotein, Hereditary persistence of, included FETA FETA_HUMAN Hereditary persistence of alpha fetoprotein HPAFP |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.