Human TNF-beta Recombinant Rabbit Monoclonal Antibody [PS01-05]
cat.: HA721242
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PS01-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein human TNF-beta. |
| Positive control: | Recombinant TNF-β protein. |
| Subcellular location: | Membrane, Secreted |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01374 Human |
| Alternative names: | LTalpha DAMA-25N12.13-004 DIF LT alpha LT LT-alpha Lta Lymphotoxin alpha (TNF superfamily, member 1) Lymphotoxin alpha Lymphotoxin-alpha MGC117668 OTTHUMP00000037612 OTTHUMP00000037613 TNF B TNF superfamily member 1 TNF, lymphocyte-derived TNF-beta TNFB TNFB_HUMAN TNFbeta TNFSF 1 TNFSF1 TNLG1E Tumor necrosis factor beta tumor necrosis factor ligand 1E Tumor necrosis factor ligand superfamily member 1 tumor necrosis factor superfamily member 1 |
Images
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Fig1:
Standard curve of TNF-β matched pair antibodies: Sandwich ELISA analysis of TNF-β matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721242 [PS01-05] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-β protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721243 [PS01-19] (Biotin, 500 ng/ml) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, recombinant TNF-β protein, Streptavidin-HRP are diluted in 1% BSA/PBST. |
|
Fig2:
Western blot analysis of Human TNF-beta on recombinant Human LTA protein (HA210742) with Rabbit anti-Human TNF-beta antibody (HA721242) at 1/2,000 dilution. Lysates/proteins at 10 ng/Lane. Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721242) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.