MALT1 Recombinant Rabbit Monoclonal Antibody [PSH02-31]
cat.: HA721244
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH02-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MALT1 aa 1-400 / 824 (Q9UDY8). |
| Positive control: | HCT 116 cell lysate, HeLa cell lysate, Ramos cell lysate, Daudi cell lysate, K-562 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, human colon tissue, HCT 116 cell lysate, Jurkat cell lysate, HepG2 cell lysate, 786-0 cell lysate, LNCaP cell lysate, PC-3M cell lysate, SW480 cell lysate, mouse lymph node tissue lysate, human colon cancer tissue. |
| Subcellular location: | Cytoplasm, perinuclear region, Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:200 |
| Uniprot #: | SwissProt: Q9UDY8 Human | Q2TBA3 Mouse |
| Alternative names: | Caspase like protein DKFZp434L132 IMD12 MALT 1 MALT associated translocation MALT lymphoma associated translocation MALT lymphoma-associated translocation Malt1 MALT1 paracaspase MALT1_HUMAN MLT 1 MLT MLT1 Mucosa associated lymphoid tissue lymphoma translocation gene 1 Mucosa associated lymphoid tissue lymphoma translocation protein 1 Mucosa-associated lymphoid tissue lymphoma translocation protein 1 Paracaspase Paracaspase-1 PCASP1 |
Images
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Fig1:
Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA721244) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Ramos cell lysate (15 µg/Lane) Lane 4: Daudi cell lysate (15 µg/Lane) Lane 5: K-562 cell lysate (15 µg/Lane) Lane 6: Mouse thymus tissue lysate (30 µg/Lane) Lane 7: Rat thymus tissue lysate (30 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721244) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MALT1 antibody (HA721244) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721244) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA721244) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: Ramos cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HepG2 cell lysate Lane 7: Daudi cell lysate Lane 8: 786-0 cell lysate Lane 9: LNCaP cell lysate Lane 10: PC-3M cell lysate Lane 11: SW480 cell lysate Lane 12: Mouse lymph node tissue lysate(no heat) Lane 13: mouse thymus tissue lysate Lane 14: rat thymus tissue lysate Cell lysates: 20ug/lane,tissue lysates: 40ug/lane Predicted band size: 92.3 kDa Observed band size: 92 kDa Exposure time: 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721244) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MALT1 antibody (HA721244) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721244) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA721244) at 1/5,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si MALT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721244) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.