Claudin 1 Recombinant Rabbit Monoclonal Antibody [PS01-43]
cat.: HA721248
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Tissue, IF-Cell
Clonality: Monoclonal
Clone number: PS01-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant full length protein within human Claudin 1.
Positive control: A431 cell lysate, HepG2 cell lysate, human tonsil tissue, human skin tissue, A431, HepG2.
Subcellular location: Cell junction, Cell membrane, Membrane, Tight junction
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  IF-Cell

1:1,000
1:1,000
1:200
1:100
Uniprot #: SwissProt: O95832 Human
Alternative names: Claudin-1 Claudin1 CLD 1 CLD1 CLD1_HUMAN CLDN 1 Cldn1 ILVASC SEMP 1 SEMP1 Senescence associated epithelial membrane protein 1 Senescence associated epithelial membrane protein Senescence-associated epithelial membrane protein
Images
HA721248_1.jpg Fig1: Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody (HA721248) at 1/1,000 dilution.

Lane 1: A431 cell lysate, 10 µg/Lane
Lane 2: HepG2 cell lysate, 10 µg/Lane

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721248) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721248_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Claudin 1 antibody (HA721248) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721248_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Claudin 1 antibody (HA721248) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721248_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Claudin 1 with Rabbit anti-Claudin 1 antibody (HA721248) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721248, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721248_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Claudin 1 with Rabbit anti-Claudin 1 antibody (HA721248) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721248, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721248_6.jpg Fig6: Immunocytochemistry analysis of A431 cells labeling Claudin 1 with Rabbit anti-Claudin 1 antibody (HA721248) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Claudin 1 antibody (HA721248) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721248_7.jpg Fig7: Immunocytochemistry analysis of HepG2 cells labeling Claudin 1 with Rabbit anti-Claudin 1 antibody (HA721248) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Claudin 1 antibody (HA721248) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.