POMC Recombinant Rabbit Monoclonal Antibody [JE32-88]
cat.: HA721250
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE32-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human POMC aa 21-70 / 267. |
| Positive control: | Rat pituitary tissue lysates, rat pituitary tissue, mouse pituitary tissue, mouse hypothalamus (arcuate nucleus) tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P01189 Human | P01193 Mouse | P01194 Rat |
| Alternative names: | ACTH Adrenocorticotropic hormone Adrenocorticotropin alpha melanocyte stimulating hormone Alpha MSH Alpha-MSH beta endorphin Beta LPH beta melanocyte stimulating hormone Beta MSH Beta-LPH Beta-MSH CLIP COLI_HUMAN Corticotropin like intermediary peptide Corticotropin lipotropin Corticotropin-lipotropin Gamma MSH Gamma-LPH Gamma-MSH Lipotropin beta Lipotropin gamma LPH Melanotropin alpha Melanotropin Melanotropin beta Melanotropin gamma met enkephalin Met-enkephalin MSH NPP POC POMC pro ACTH endorphin Proopiomelanocortin Proopiomelanocortin preproprotein |
Images
|
Fig1:
Application: IF-tissue Species: Mouse Site: Pituitary Sample: Paraffin-embedded section Antibody concentration: 1:1,000 |
|
Fig2:
Application: IF-tissue Species: Mouse Site: Hypothalamus (arcuate nucleus) Sample: Paraffin-embedded section Antibody concentration: 1:1,000 |
|
Fig3:
Western blot analysis of POMC on rat pituitary tissue lysates with Rabbit anti-POMC antibody (HA721250) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721250) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-POMC antibody (HA721250) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-POMC antibody (HA721250) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.