KDM3B Recombinant Rabbit Monoclonal Antibody [PSH0-11]
cat.: HA721252
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 191 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KDM3B aa 251-500. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, Hela. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q7LBC6 Human | Q6ZPY7 Mouse |
| Alternative names: | 5qNCA C5orf7 JHDM2B JmjC domain containing histone demethylation protein 2B JmjC domain-containing histone demethylation protein 2B jmjd1b Jumonji domain containing 1B Jumonji domain containing protein 1B Jumonji domain-containing protein 1B KDM3B KDM3B_HUMAN KIAA1082 Lysine (K) specific demethylase 3B Lysine-specific demethylase 3B NET22 Nuclear protein 5qNCA |
Images
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Fig1:
Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA721252) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Predicted band size: 191 kDa Observed band size: 191 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721252) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA721252) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA721252) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.