CD116 Recombinant Rabbit Monoclonal Antibody [JE61-37]
cat.: HA721253
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE61-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD116 aa 23-122 / 400. |
| Positive control: | JAR cell lysate, Hela cell lysate, 293T cell lysate, human placenta tissue lysate, human malignant melanoma tissue, human skin tissue, SiHa. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:1,000 1:50 |
| Uniprot #: | SwissProt: P15509 Human |
| Alternative names: | CD_antigen=CD116 CD116 CD116 antigen CDw116 Colony stimulating factor 2 receptor alpha chain Colony stimulating factor 2 receptor alpha low affinity Colony stimulating factor 2 receptor alpha subunit CSF 2R CSF2R CSF2R_HUMAN CSF2RA CSF2RAX CSF2RAY CSF2RX CSF2RY GM CSF R alpha GM CSF receptor alpha subunit GM-CSF-R-alpha GMCSFR GMCSFR-alpha GMR-alpha Granulocyte macrophage colony stimulating factor receptor alpha chain Granulocyte-macrophage colony-stimulating factor receptor subunit alpha SMDP4 |
Images
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Fig1:
Western blot analysis of CD116 on different lysates with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution. Lane 1: JAR cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: Human placenta tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721253) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SiHa cells labeling CD116 with Rabbit anti-CD116 antibody (HA721253) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD116 antibody (HA721253) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.