WDR77 Recombinant Rabbit Monoclonal Antibody [PSH0-07]
cat.: HA721260
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: PSH0-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within human WDR77 aa 1-342 / 342.
Positive control: Human prostate tissue, human fallopian tube tissue, mouse brain tissue, HepG2 cell lysate, 293 cell lysate, K562 cell lysate, Hela, HepG2.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000
1:1,000
1:500-1:1,000
Uniprot #: SwissProt: Q9BQA1 Human
Alternative names: 2610312E17Rik Androgen receptor cofactor p44 C79984 HKMT1069 MEP 50 MEP-50 MEP50 MEP50_HUMAN Methylosome protein 50 MGC2722 Nbla10071 p44 p44/Mep50 RGD1310479 RP11 552M11.3 WD repeat containing protein 77 WD repeat domain 77 WD repeat-containing protein 77 WDR77
Images
HA721260_1.jpg Fig1: Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: 293 cell lysate
Lane 3: K562 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 40 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721260_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721260_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721260_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721260_5.jpg Fig5: Flow cytometric analysis of Hela cells labeling WDR77.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721260, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721260_6.jpg Fig6: Flow cytometric analysis of HepG2 cells labeling WDR77.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721260, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.