WDR77 Recombinant Rabbit Monoclonal Antibody [PSH0-07]
cat.: HA721260
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WDR77 aa 1-342 / 342. |
| Positive control: | Human prostate tissue, human fallopian tube tissue, mouse brain tissue, HepG2 cell lysate, 293 cell lysate, K562 cell lysate, Hela, HepG2. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9BQA1 Human |
| Alternative names: | 2610312E17Rik Androgen receptor cofactor p44 C79984 HKMT1069 MEP 50 MEP-50 MEP50 MEP50_HUMAN Methylosome protein 50 MGC2722 Nbla10071 p44 p44/Mep50 RGD1310479 RP11 552M11.3 WD repeat containing protein 77 WD repeat domain 77 WD repeat-containing protein 77 WDR77 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of Hela cells labeling WDR77. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721260, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of HepG2 cells labeling WDR77. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721260, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (HA721260) at 1/1,000 dilution. Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 16 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721260, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.