VILIP1 Recombinant Rabbit Monoclonal Antibody [PSH0-08]
cat.: HA721261
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH0-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within human VILIP1 aa 1-191 / 191.
Positive control: Human liver tissue lysate, Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue, human brain tissue, mouse brain tissue.
Subcellular location: Cytosol, membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: P62760 Human | P62761 Mouse | P62762 Rat
Alternative names: 21 kDa CABP Hippocalcin like protein 3 Hippocalcin-like protein 3 HLP 3 HLP3 HPCAL 3 HPCAL3 HUVISL1 Neural visinin-like protein 1 Neural visinin-like type 1 protein Neurocalcin alpha NVL 1 NVL1 Nvp1 OZ1 Ratnvp1 VILIP VILIP-1 Visinin Visinin like 1 Visinin like protein 1 Visinin-like protein 1 VISL 1 VISL1 VISL1_HUMAN VLP-1 Vnsl1 Vsnl1
Images
HA721261_1.jpg Fig1: Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution.

Lane 1: Human liver tissue lysate (20 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Lane 3: Hela cell lysate (10 µg/Lane)

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721261_2.jpg Fig2: Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721261_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721261_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721261_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.