VILIP1 Recombinant Rabbit Monoclonal Antibody [PSH0-08]
cat.: HA721261
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human VILIP1 aa 1-191 / 191. |
| Positive control: | Human liver tissue lysate, Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue, human brain tissue, mouse brain tissue. |
| Subcellular location: | Cytosol, membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P62760 Human | P62761 Mouse | P62762 Rat |
| Alternative names: | 21 kDa CABP Hippocalcin like protein 3 Hippocalcin-like protein 3 HLP 3 HLP3 HPCAL 3 HPCAL3 HUVISL1 Neural visinin-like protein 1 Neural visinin-like type 1 protein Neurocalcin alpha NVL 1 NVL1 Nvp1 OZ1 Ratnvp1 VILIP VILIP-1 Visinin Visinin like 1 Visinin like protein 1 Visinin-like protein 1 VISL 1 VISL1 VISL1_HUMAN VLP-1 Vnsl1 Vsnl1 |
Images
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Fig1:
Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VILIP1 antibody (HA721261) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721261) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.