CENPA Recombinant Rabbit Monoclonal Antibody [PSH0-09]
cat.: HA721262
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CENPA aa 21-70 / 140. |
| Positive control: | Hela cell lysate, A375 cell lysate, HeLa. |
| Subcellular location: | Nucleus, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P49450 Human |
| Alternative names: | CENP A CENP-A cenpa CENPA_HUMAN Centromere autoantigen A Centromere protein A 17kDa Centromere protein A Histone H3 like centromeric protein A Histone H3-like centromeric protein A |
Images
|
Fig1:
Western blot analysis of CENPA on different lysates with Rabbit anti-CENPA antibody (HA721262) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A375 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721262) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling CENPA with Rabbit anti-CENPA antibody (HA721262) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 2% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CENPA antibody (HA721262) at 1/100 dilution in 0.1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.