HA721263

PSME2 Recombinant Rabbit Monoclonal Antibody [PSH0-10]

cat.: HA721263

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	PSH0-10

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 27 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human aa 2-239.
Positive control:	PC-12 cell lysate, 293T cell lysate, mouse liver tissue lysate, rat thymus tissue lysate, MCF-7 cell lysate, PC-3 cell lysate, Hela, rat stomach tissue, human rectal carcinoma tissue, HepG2.
Subcellular location:	Cytosol, Extracellular region or secreted, Nucleus.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000-1:5,000 1:100 1:4,000 1:500-1:1,000
Uniprot #:	SwissProt: Q9UL46 Human \| P97372 Mouse \| Q63798 Rat
Alternative names:	11S regulator complex beta subunit 11S regulator complex subunit beta Activator of multicatalytic protease subunit 2 Cell migration inducing protein 22 MCP activator 31 kD subunit PA28 beta PA28b PA28beta Proteasome (prosome macropain) activator subunit 2 (PA28 beta) Proteasome (prosome macropain) activator subunit 2 Proteasome activator 28 beta Proteasome activator 28 subunit beta Proteasome activator complex subunit 2 Proteasome activator hPA28 subunit beta Proteasome activator subunit 2 PSME 2 PSME2 PSME2_HUMAN REG beta REG-beta REGbeta

Images

Fig1: Western blot analysis of PSME2 on different lysates with Rabbit anti-PSME2 antibody (HA721263) at 1/2,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si PSME2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 27 kDa
Observed band size: 30 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721263) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PSME2 on different lysates with Rabbit anti-PSME2 antibody (HA721263) at 1/1,000 dilution.

Lane 1: PC-12 cell lysate (10 µg/Lane)
Lane 2: 293T cell lysate (10 µg/Lane)
Lane 3: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Rat thymus tissue lysate (20 µg/Lane)
Lane 5: MCF-7 cell lysate (10 µg/Lane)
Lane 6: PC-3 cell lysate (10 µg/Lane)

Predicted band size: 27 kDa
Observed band size: 30 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721263) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunocytochemistry analysis of Hela cells labeling PSME2 with Rabbit anti-PSME2 antibody (HA721263) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSME2 antibody (HA721263) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PSME2 antibody (HA721263) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721263) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human rectal carcinoma tissue with Rabbit anti-PSME2 antibody (HA721263) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721263) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Flow cytometric analysis of Hela cells labeling PSME2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721263, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Fig7: Flow cytometric analysis of HepG2 cells labeling PSME2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721263, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.