ISG15 Recombinant Rabbit Monoclonal Antibody [JE32-27]
cat.: HA721264
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE32-27
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of Human ISG15.
Positive control: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate.
Subcellular location: Cytoplasm, Secreted.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P05161 Human
Alternative names: G1P2 hUCRP IFI 15 IFI15 Interferon alpha inducible protein Interferon induced protein 15 Interferon induced protein IFI-15K Interferon stimulated protein 15 kDa Interferon, alpha-inducible protein (clone IFI-15K) Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Interferon-induced 17-kDa/15-kDa protein IP17 ISG 15 ISG15 ISG15 ubiquitin like modifier ISG15_HUMAN Ubiquitin cross-reactive protein Ubiquitin-like protein ISG15 UCRP
Images
HA721264_1.jpg Fig1: Western blot analysis of ISG15 on different lysates with Rabbit anti-ISG15 antibody (HA721264) at 1/500 dilution.

Lane 1: Hela whole cell lysate
Lane 2: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 18 kDa
Observed band size: 13 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721264) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.