ISG15 Recombinant Rabbit Monoclonal Antibody [JE32-27]
cat.: HA721264
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE32-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Human ISG15. |
| Positive control: | Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate. |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P05161 Human |
| Alternative names: | G1P2 hUCRP IFI 15 IFI15 Interferon alpha inducible protein Interferon induced protein 15 Interferon induced protein IFI-15K Interferon stimulated protein 15 kDa Interferon, alpha-inducible protein (clone IFI-15K) Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Interferon-induced 17-kDa/15-kDa protein IP17 ISG 15 ISG15 ISG15 ubiquitin like modifier ISG15_HUMAN Ubiquitin cross-reactive protein Ubiquitin-like protein ISG15 UCRP |
Images
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Fig1:
Western blot analysis of ISG15 on different lysates with Rabbit anti-ISG15 antibody (HA721264) at 1/500 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721264) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.