Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE32-27 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 18 kDa |
Isotype: | IgG |
Immunogen: | Recombinant full length protein of Human ISG15. |
Positive control: | Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate. |
Subcellular location: | Cytoplasm, Secreted. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P05161 Human |
Alternative names: | G1P2 hUCRP IFI 15 IFI15 Interferon alpha inducible protein Interferon induced protein 15 Interferon induced protein IFI-15K Interferon stimulated protein 15 kDa Interferon, alpha-inducible protein (clone IFI-15K) Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Interferon-induced 17-kDa/15-kDa protein IP17 ISG 15 ISG15 ISG15 ubiquitin like modifier ISG15_HUMAN Ubiquitin cross-reactive protein Ubiquitin-like protein ISG15 UCRP |
Fig1:
Western blot analysis of ISG15 on different lysates with Rabbit anti-ISG15 antibody (HA721264) at 1/500 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721264) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |